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Objective To investigate the clinical manifestations and imaging features of isolated cortical vein thrombosis (ICoVT).Methods The data of patients with ICoVT admitted and treated in Shengjing Hospital of China Medical University from 2005 to 2015 were collected.Their clinical manifestations and imaging features were analyzed.Results A total of 104 patients with cerebral venous sinus thrombosis were enrolled.Seven (6.7％) of them with ICoVT were enrolled in the analysis.There were 6 women (85.7％).Their average age was 35 years (range 25-46 years).Headache (n =6;85.7％),seizures (n=5;71.4％),and limb paralysis and numbness (n =4;57.1％) were the most common clinical manifestations.All patients underwent head CT,MRI,and magnetic resonance venography (MRV).Focal cerebral tissue edema (n =5;71.4％) and hemorrhagic infarction (n =3;42.9％) were the most common imaging features.Susceptibility weighted imaging of 3 cases revealed cord-like hypointensity,indicating the formation of venous thrombosis.The clinical symptoms of all patients were relieved after anticoagulant therapy.They were followed up for 1.5 years and no recurrence was observed.Conclusion Headache and seizures are the most common clinical symptoms of IcoVT.Imaging is mostly manifested as focal cerebral edema or hemorrhagic infarction.
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Objective To investigate the evaluation index of melasma staging by clinical manifestations and non-invasive skin detection technology.Methods A total of 195 patients with a clinical diagnosis of melasma were enrolled from the First Affiliated Hospital of Kunming Medical University.The skin with lesion enlarged,color darker,erythema,red occured after scratching or lesion faded after compressing with glass belonged to the active stage;on the contrary,it was in the stable stage.Reflectance confocal microscopy (RCM),dermoscopy,Mexameter 18 and LAB were used to observe skin lesions of different stage of melasma.Results There were 115 patients (59.0 ％) in the active stage of melasma and 80 patients (41.0 ％) in the stable stage.DMA score in active stage 35.08± 10.59 were significantly higher than that of the stable stage 15.06-4-9.20 (P＜0.05).There were statistically significant difference in the quantity of inflammatory cell and blood vessels between two stages of melasma (P＜0.05).Erythema index (EI) in active stage of melasma 376.35±61.39 were high-er than that of the stable stage 320.364± 62.40 (P＜0.05).A-value in active stage of melasma 13.28± 1.75 were higher than that of the stable stage 12.34± 1.78 (P＜0.05).However,there were no siginificant differences in the quantity of melenin,melanin index (MI),L-value and B-value.Conclusions Melasma could be divided into active stage or stable stage,respectively,according to its clinical manifestations.DMA score,quantity of inflammatory cells and blood vessels,EI and A-value could be used as the reference index of melasma staging.
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Objective To investigate the effect of astaxanthin on the peripheral blood endothelial progenitor cells (EPCs) apop-tosis induced by oxidative stress in vitro and to explore its underlying mechanism .Methods Human peripheral blood EPCs were in vitro cultured and divided into the control group ,model group with 100 μmol/L tert-butyl hydroperoxide(tBHP) and the astaxan-thin plus tBHP group(with 0 .10 ,1 .00 ,10 .00 nmol/L astaxanthin pretreatment for 24 h ,then adding the final concentration of 100μmol/L tBHP for 6 h continuous culture) .The cell viability was measured by the MTT method .The level of reactive oxygen spe-cies (ROS) was determined by the DCFH-DA method .The changes of mitochondrial membrane potential(MMP) and the apoptosis ratio were detected by the JC-1 method and the DAPI method ,respectively .Results Compared with control group ,100μmol/L tB-HP could obviously caused the apoptosis of EPCs(P<0 .05) ,while astaxanthin could decrease tBHP induced apoptosis ,which man-ifested by the decrease of the apoptosis ratio (P<0 .05) and MMP increase .Conclusion Astaxanthin has the protective effect on the apoptosis of EPCs ,its mechanism may be related with the protection of the mitochondrial function .
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Automation workstation refers to a kind of fully automatic platform,which use the automatic technology to analysis,processe batches of biological samples.It can finish liquid handling,transfering,mixing and other operatious in short time,particularly suitable for PCR templates'preparation of large amount of biological samples.This article mainly discusses the basic patterns,methods and forensic applications of automated DNA extraction workstation,aimed at making the technology more widely applied in forensic DNA analysis.